User-based (Unassisted) Flow Cytometric Analysis: Equipment is available 24/7 for trained investigators. During regular working hours, operators are available to assist with setup, running samples and trouble-shooting.
Assisted Flow Cytometric Analysis: An operator is available for the Fortessa X-20, LSRFortessa and FACSVerse during regular working hours to provide general assistance as well as standard assays including cell death, proliferation, cell cycle, Ca++ flux, etc.
Cell Sorting:The FCCS offers high-speed cell sorting services for isolation of complex and rare cell populations. The staff of the FCCS has considerable expertise in the design and implementation of novel methodologies for cell/particle isolation and is available to work with investigators to support study goals. Cell sorting is provided as an operator-based assisted service.
Mass Cytometry:The FCCS provides mass cytometry services for high-dimensional single-cell analysis. The Helios (CyTOF) platform is capable of simultaneous analysis of up to 135 antigens with current studies aimed at 45-50 parameter detection. Dr. Krieg provides expertise in experimental design, data analysis, and troubleshooting for CyTOF experiments. Mass cytometry analysis is provided as an operator-based assisted service.
If you publish a manuscript including research supported by the Flow Cytometry & Cell Sorting Shared Resource, the HCC recommends the following text to be placed in the acknowledgement section: "Supported in part by the Cell Evaluation & Therapy Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."