Flow Cytometry & Cell Sorting Unit


Associate
Director
Amanda C. LaRue, Ph.D.
Associate Professor,
Pathology & Laboratory Medicine
Operations
Manager
Adam Soloff, Ph.D.
Research Assistant Professor,
Microbiology & Immunology
843-792-4794 | soloff@musc.edu
Mass Cytometry Operations Manager
Carsten Krieg, Ph.D.
Assistant Professor of Microbiology & Immunology and Dermatology
843-792-9995 | kriegc@musc.edu
Research
Specialist
Corinne Levingston
843-792-8115
Hollings Cancer Center - 324
Research
Specialist
Kirstin McDanel
843-792-8115
Hollings Cancer Center - 324

Services

User-based (Unassisted) Flow Cytometric Analysis: Equipment is available 24/7 for trained investigators. During regular working hours, operators are available to assist with setup, running samples and trouble-shooting.

Assisted Flow Cytometric Analysis: An operator is available for the Fortessa X-20, LSRFortessa and FACSVerse during regular working hours to provide general assistance as well as standard assays including cell death, proliferation, cell cycle, Ca++ flux, etc.

Cell Sorting:The FCCS offers high-speed cell sorting services for isolation of complex and rare cell populations. The staff of the FCCS has considerable expertise in the design and implementation of novel methodologies for cell/particle isolation and is available to work with investigators to support study goals. Cell sorting is provided as an operator-based assisted service.

Mass Cytometry:The FCCS provides mass cytometry services for high-dimensional single-cell analysis. The Helios (CyTOF) platform is capable of simultaneous analysis of up to 135 antigens with current studies aimed at 45-50 parameter detection. Dr. Krieg provides expertise in experimental design, data analysis, and troubleshooting for CyTOF experiments. Mass cytometry analysis is provided as an operator-based assisted service.

Additional Information & Helpful Links

Publication Acknowledgement

If you publish a manuscript including research supported by the Flow Cytometry & Cell Sorting Shared Resource, the HCC recommends the following text to be placed in the acknowledgement section: "Supported in part by the Cell Evaluation & Therapy Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."

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