Confocal & Multiphoton Microscopy Unit


Associate Director
Anna-Liisa Nieminen, Ph.D.
Associate Professor,
Drug Discovery & Biomedical Sciences
nieminen@musc.edu
Manager
Monika Beck Gooz, M.D., Ph.D.
Research Associate Professor,
Drug Discovery & Biomedical Sciences
843-876-2363
Imaging Specialist

Li Li, Ph.D.
Drug Discovery Building 522B
843-876-2358

Applications

  • Fixed Cell & Tissue Imaging using chromogenic and fluorescence techniques in combination with antibody-based methods to monitor expression, distribution and interaction of specific molecules.

  • Live Cell Imaging of parameter-sensitive fluorophores to monitor ions, electrical potentials, oxygen and nitrogen radical generation, NAD(P)H, mitochondrial and plasmalemmal membrane permeability, cell viability (apoptosis and necrosis), fluorescent protein biosensors and other parameters.

  • Intravital Microscopy to monitor microcirculation, leukocyte margination, invadopodia, mitochondrial polarization, membrane permeability, radical generation, gene expression, detection of collagen fibers and other factors in living animals and tissues.

  • Advanced Imaging Techniques: Fluorescence resonance energy transfer (FRET) and fluorescence recovery after photobleaching (FRAP) to characterize and quantify interactions between specific molecules and their mobility; second harmonic generation (SHG) microscope for label-free visualization of collagen; Airyscan super-resolution imaging with high quantum efficiency GaAsP photomultipliers.

Services & Equipment

  • Zeiss 880 LSM NLO with Airyscan Super-resolution Detector: Confocal/multiphoton microscope (DD 521D) for long-term fluorescence imaging of live cells, embryos and tissues of living organisms as well as fixed cells and tissues.

  • Olympus FV1200 MPE: Intravital multiphoton microscope (DD 521C) for imaging live animals and thick specimens.

  • Zeiss LSM 510 META: Confocal microscope (HCC 355) for fluorescence imaging of live cells and fixed specimens.

  • Olympus FluoView FV10i LIV: Confocal microscope (DD 521B) for automated long-term fluorescence imaging of live cells, as well as fixed specimens.

  • BD Biosciences CARV II: Disk-screening confocal microscope (DD 526) for bright-field and both widefield and confocal fluorescence imaging.

Full Microscope Descriptions
  • Imaging Workstation
    An imaging workstation is dedicated for off-line image processing and analysis. The following softwares are available for current CMI users: Metamorph, Image J FIJI, Duolink ImageTool, Adobe Photoshop, Olympus Viewer, IP Lab and Zeiss Zen software. Currently, no charges are assessed for computer use. Please contact Monika Beck Gooz, M.D., Ph.D. for more information and to request training on the various softwares.

  • Live Cell Imaging
    A tissue culture hood (37°C) and two cell culture incubators are available in Drug Discovery 521 for specimen preparation and temporary incubation of cells. One incubator is equipped for 5 percent CO2.

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