shRNA Order Request

Step 1: Finding shRNAs of Interest

Prior to ordering, the requestor will need to identify the TRCN number for the shRNA clones targeting their gene or genes of interest. Please follow these guidelines in order to identify the shRNA sequences you require:

• Visit the Sigma Mission shRNA library website.
• Select your favorite TM gene search.
• Enter gene or gene symbol.
• Check correct gene is chosen and from "products" select "shRNA."
• From the search window that appears, select mouse or human Mission shRNA bacterial glycerol stock.
• From the list that appears, select the shRNA sequences you require and make a note of the TRCN numbers for ordering.

Some of the shRNA sequences have already been validated by Sigma and are indicated. It is advised to identify where each sequence is located especially if isoforms exist for your target of interest. If we do not have the clones specified, we will try to provide alternative options.

We recommend sequencing all vectors to confirm the insert is correct as early as possible.

Step 2: Ordering shRNAs of Interest

Please Note: All requests must be supported by a current investigator IBC# covering the target genes of interest and the use of lentiviral particles.

The shRNA Technology Shared Resource uses Infinity to receive order requests and streamline the billing process. If you are already registered with Infinity, you can use your existing user information.