Short hairpin RNA’s (shRNAs) can efficiently suppress gene expression in human cells. The shRNA Technology Shared Resource will provide investigators at MUSC access to genome wide human and mouse libraries that together encode a total of almost 160,000 shRNA clones against over 41,000 genes. The resource utilizes The RNAi Consortium’s (TRC) genome-wide lentiviral mouse and human libraries and investigators will have the option of ordering shRNA’s targeting single or multiple genes, gene family sets as well as pathway specific pooled libraries. The library will allow access to multiple shRNAs for a single gene which is important for validation against off target effects. This technology holds tremendous power, and is ready to help investigators at MUSC work toward breakthrough discoveries.
Human Library: 20,018 genes, 129,695 clones (1500-96 well plates).
Mouse Library: Mouse Library: 21,171 genes, 118,062 clones (1374-96 well plates).
Protocols