Small Animal Imaging

The Small Animal Imaging unit provides imaging support for biomedical research and is located on the 4th floor of Hollings Cancer Center in suite 403. Access to the imaging space and all equipment must be approved by the facility director. Users will be allowed access to the imaging space by the facility manager once they have completed a training session. Please see our general policies for more information about use of the equipment.

Bioluminescence Imaging

Small animal bioluminescence imaging provides state-of-the-art noninvasive visualization of luciferase-expressing tissues. The equipment monitors cellular events such as tumor progression and metastasis in living animals. In vivo bioluminescence imaging is also used to evaluate tumor metabolism and cell biology in response to genetic manipulations, pharmacologic agents and cancer chemotherapy drugs.

Equipment

PerkinElmer Xenogen IVIS 200 In Vivo Bioluminescence Imaging System

The IVIS 200 is capable of imaging up to five animals at a time and provides limited 3-D depth information. The imaging system includes a heated specimen platform for the animals and a cooled back-thinned CCD camera to capture both visible light photographs and luminescent images. Images can be obtained in less than one minute. Users must undergo a one-time training session prior to being able to schedule use.

Staff

Associate Director
John O’Bryan, Ph.D.
Professor, Cell and Molecular Pharmacology & Experimental Therapeutics
843-792-8393
obryanjo@musc.edu

Equipment Managers
Imran Khan, Ph.D.
Staff Scientist, Cell and Molecular Pharmacology & Experimental Therapeutics
843-792-2277
khanim@musc.edu

J. Matthew Rhett, Ph.D.
Staff Scientist, Cell and Molecular Pharmacology & Experimental Therapeutics
843-792-6837
rhettj@musc.edu

Pricing

There is a charge of $40 per hour for training and use of the IVIS imaging system. If you schedule training or equipment usage, it is assumed that you have approval from your PI for the charges.

Request IVIS Training

A training session is required for new users. Please use the form at the link below to submit a request for training, and a staff member will respond to schedule a time.

Schedule IVIS Use

If you have already completed training, please use the form at the link below to schedule use of the IVIS imaging system.

To check times that the IVIS is available, view the IVIS-Small Animal Imaging shared calendar in Outlook. Use our Adding a Shared Calendar guide (PDF) for step-by-step instructions.

To cancel a previously scheduled time, forward the confirmation email you received to hcc-ivisequipment@musc.edu. If you need to cancel less than 4 hours prior, contact the facility staff directly.

Fluorescence Imaging

Small animal fluorescence imaging provides state-of-the-art fluorescence imaging of small animals and can generate anatomical organ maps for co-localization. This allows for imaging of fluorescence-labeled cells (e.g. GFP expressing cancer cells), fluorescent drugs (e.g. anthracyclines), and other compounds tagged by fluorescent probes.

Equipment

PerkinElmer Maestro 2 In Vivo Fluorescence Imaging System

The Maestro 2 features a light-tight enclosure with access ports (e.g. for imaging during injection in catheterized animals), configurable imaging area, tunable multispectral camera, heated imaging chamber, and spectral unmixing at the following wavelengths:

  • 6 excitation filters: 470 nm (blue) – 740 nm (near infrared)
  • 45 emission filters: 500 nm (green) – 950 nm (near infrared) in 10 nm increments

Staff

Dieter Haemmerich, Ph.D.
Professor, Pediatrics
843-792-1396
haemmer@musc.edu

Pricing

There is no charge to use the Maestro imaging system.

Schedule Maestro Use

Please use the form at the link below to request a time to use the Maestro imaging system. A training session is required for new users. Please select "I need training" if this will be your first time using the equipment.

To check times that the Maestro is available, view the Maestro-Small Animal Imaging shared calendar in Outlook. Use our Adding a Shared Calendar guide (PDF) for step-by-step instructions.

To cancel a previously scheduled time, forward the confirmation email you received to hccmaestroequipment@musc.edu. If you need to cancel less than 4 hours prior, contact the facility staff directly.

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