Equipment

Zeiss 880 LSM NLO

Drug Discovery - 521D: confocal/multiphoton microscope for long-term fluorescence imaging of live cells, embryos and tissues of living organisms as well as fixed cells and tissues

  • Confocal fluorescence imaging using single and multiphoton laser excitation
  • Axio Observer Z1 inverted microscope equipped with 10X, 25X, 40X and 63X objectives
  • Visible lasers for confocal excitation at 405, 458, 477, 488, 514, 561 and 633 nm
  • Coherent Chameleon Ultra Ti:Sapphire multiphoton laser tunable from 690-1080 nm
  • Multiphoton fluorescence imaging in both descanned and non descanned modes

Olympus FV1200 MPE

Drug Discovery - 521C: intravital multiphoton microscope for imaging live animals and thick specimens

  • Olympus IX83 inverted microscope stand
  • 4X and 10X air objectives plus 30X and 60X long working distance silicone oil immersion objectives
  • Brightfield and DIC imaging using two photon excitation
  • Spectraphysics MaiTai DeepSee multiphoton laser tunable from 690-1040nm

Zeiss LSM 510 META

Hollings Cancer Center - 355: confocal microscope for fluorescence imaging of live cells and fixed specimens

  • Confocal fluorescence imaging
  • Axiovert 200M inverted microscope stand equipped with 10X, 25X and 63X objectives
  • Brightfield and DIC imaging
  • Laser excitation at 477, 488, 514, 543 and 633 nm

Olympus FluoView FV10i LIV

Drug Discovery - 521B: confocal microscope for automated long-term fluorescence imaging of live cells, as well as fixed specimens

  • 10X air and 60X water immersion objectives
  • Phase contrast imaging
  • Laser excitation at 405, 473, 559 and 635 nm

BD Biosciences CARV II

Drug Discovery - 526: disk-screening confocal microscope for brightfield and both widefield and confocal fluorescence imaging

  • Zeiss Axiovert 200M inverted microscope stand
  • 10X, 20X air and 40X and 60X oil immersion objectives
  • Brightfield imaging of histochemical and immunohistochemical samples

 

  • Brightfield and DIC imaging using single and multiphoton excitation
  • Spectral analysis and unmixing using Quasar 32-channel photomultiplier array
  • GaAsP detectors for highest sensitivity imaging
  • Simultaneous 3-channel fluorescence imaging and DIC
  • Other imaging modes include time-lapse, multi-track, intravital, depth, ratio, PLIM (phosphorescence lifetime imaging), FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer) and second harmonic generation
  • Stage top incubator for temperature and CO2 control with objective heater
  • Zeiss LSM 880 Operating Instructions (PDF)

 

  • Spectral analysis and unmixing with META photomultiplier array
  • Simultaneous multi-channel image acquisition with multi-tracking
  • Other imaging modes include time-lapse, multi-track, depth, ratio, FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer)
  • Stage top incubator for temperature and CO2 control with objective heater
  • Zeiss LSM 510 User Manual (PDF)

    Zeiss LSM 510 Operating Instructions (PDF)

  • Detection through a variable bandpass filter for selection of the most suitable wavelength for a particular fluorophore
  • Environmental control for live cell imaging, including hypoxia control
  • Z-drift compensation function for long-term imaging
  • Olympus FV10i User Manual (PDF)

    Olympus FV10i Operating Instructions (PDF)

 

Back to top Back to top