The mission of the Lipidomics Shared Resource is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and synthetic molecular tools) to enhance understanding of the role of bioactive lipids in cell (cancer) biology with the goal to discover future drugs. The Lipidomics Shared Resource is organized into two entities, the Synthesis Unit and the Analytical Unit.
Analytical Unit - 505C Children's Research Institute
Synthesis Unit - 748/747B - Basic Science Building
Services & Expertise
The Lipidomics Shared Resource is designed to support research in lipidomics by providing advanced methodology, state-of-the-art facilities and expertise for both synthesis and analysis of bioactive lipids in a cost-effective manner.
This resource provides the following services with expertise in sphingolipid chemistry, analysis and biology:
- Qualitative and quantitative analysis of sphingoipid components at the metabolomics level from biological samples (cells, tissue, biological fluids) and determination of protein and total phospholipids in biological samples used for data normalization.
- Homogeneous sphingolipid standards, molecular tools to study sphingolipid metabolism, and diversified synthetic sphingolipids and analogs for cellular, in vitro and in vivo studies.
- Assistance in experimental design, selection of lipid of interest, and interpretation of analytical data.
The Lipidomics Shared Resource focuses on the following key areas:
- Providing synthetic lipids, analogs, and inhibitors of lipid metabolism with an emphasis on sphingolipids.
- Developing cutting-edge synthetic molecular tools to study the role of bioactive lipids.
- Developing lead compounds for translational studies. The Lipidomics Shared Resource’s library consists of over 750 synthetic lipids, derivatives and related molecules.
- Providing qualitative and quantitative analysis of lipid composition from biological materials with a current listing of more than 350 distinct molecular species covering sphingolipid metabolism at a basic metabolomic level (from sphingoid bases to complex phospho- and glyco-sphingolipids).
- Providing and developing new methods for determination of cellular enzymatic activities using the LC-MS/MS approach.
- Improving and developing new techniques for extended lipid analyses utilizing a database of the lipid composition of normal and transformed cells and organelles that will permit a lipidomics approach to analysis of their cellular metabolism.
- Educating and assisting lipidomics investigators in designing and conducting experimental approaches.
The following text must be placed under the Acknowledgements Section of publications, presentations, posters, reports, etc. that uses data or reagents generated by the Lipidomics Shared Resource:
“Research was supported in part by the Medical University of South Carolina’s Lipidomics Shared Resource; for Hollings Cancer Center (P30 CA138313), the Lipidomics Shared Resource in the South Carolina Lipidomics and Pathobiology COBRE; MUSC Department of Biochemistry (P20 RR017677), and the National Center for Research Resources and Office of the Director of the National Institutes of Health (C06 RR018823) for the occupancy of space for the Lipidomics Shared Resource, Analytical Unit in 505 Children’s Research Institute (CRI).”