Lipidomics Shared Resource

The Lipidomics Shared Resource at MUSC represents expertise specializing in sphingolipid biology, providing services to the Medical University of South Carolina and institutions or industries throughout the world. The Lipidomics Shared Resource is composed of analytical and synthetic units providing expertise, synthetic compounds/standards, and analytical methodology (LC-MS/MS) to enhance an understanding of the role of bioactive lipids in cell (cancer) biology.


Director
Besim Ogretmen, Ph.D.
Professor, Biochemistry & Molecular Biology
ogretmen@musc.edu | 843-792-0940
Shared Resource Locations
Analytical Unit: 505C Children's Research Institute
Synthesis Unit: 748 Basic Science Building

Overview

The Lipidomics Shared Resource builds on the unique expertise of key personnel in sphingolipid chemistry, analysis, and biology providing intellectual and physical resources to enhance the understanding of bioactive sphingolipids and lipids in signal transduction and cell regulation.

Sphingolipids are recognized as key components regulating fundamental cell biology processes regulating transmembrane signaling. Diversity of bioactive sphingolipids and their interconnected metabolism provide a network of pathways. Key sphingolipid metabolites are: ceramides (Cer), sphingosine (Sph), sphingosine 1-phosphate (S1P), and ceramide 1-phosphate (Cer1P).

Services & Expertise

The Lipidomics Shared Resource is designed to support research in lipidomics by providing advanced methodology and expertise for both synthesis and analysis of bioactive lipids in a cost-effective manner, with a focus on several key areas:

  • Providing qualitative and quantitative analysis of >300 sphingolipids using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technologies.
  • Providing analytical method development of new lipid components.
  • Providing and developing synthetic lipid tools/standards, including functionalized and fluorescent ceramides, 17C-sphingolipids; or synthetic lipid analogs, including organelle-targeted sphingolipids.
  • Mentoring investigators in the understanding of lipid biology through lipid chemistry, experimental design, selection of lipids of interest, and interpretation of the analytical data.

Publication Acknowledgement

The following text must be placed under the Acknowledgements section of publications:

“Supported in part by the Lipidomics Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313 and P30 GM103339)”.