7th Charleston Workshop on Light Microscopy for Biosciences - June 9-14, 2019
Sunday, June 9
| Time |
Event |
| 12:00 p.m. |
Registration – VMB125 |
| 1:00 p.m. |
Course Introduction – VMB125 |
| 1:30 p.m. |
Lecture 1: Imaging and Illumination Light Paths in the Microscope, John J. Lemasters –VMB125 |
| 3:00 p.m. |
Lab 1: Wide Field Microscopy: Protocols 1-6 – DD112 (Pharmacy Lab) |
| 6:00 p.m. |
Pizza party and introductions – DD511 (GE Healthcare) |
Monday, June 10
| Time |
Event |
| 8:30 a.m. |
Lecture 2: Image Formation, Phase Contrast, Polarization and DIC, Lemasters – VMB125 |
| 9:30 a.m. |
Coffee Break |
| 10:00 a.m. |
Lab 2: Wide field microscopy: Protocols 7-10 –DD112 |
| 12:00 p.m. |
Lunch (own your own) |
| 1:00 p.m. |
Lecture 3: Principles of Fluorescence Microscopy, Anna-Liisa Nieminen |
| 2:00 p.m. |
Coffee Break |
| 2:30 p.m. |
Lab 3: Wide field microscopy: Protocols 11-13 |
| 4:00 p.m. |
Lecture 4: Filters for Fluorescence Microscopy, Mike Stanley, Chroma |
| 4:30 pm |
Lecture 5: High content imaging, BioTek |
| 5:00 p.m. |
Dinner (own your own) |
Tuesday, June 11
| Time |
Event |
| 8:30 a.m. |
Lecture 6: Digital cameras, Butch Granada, Hamamatsu – VMB125 |
| 9:30 a.m. |
Lecture 7: Fluorescence Probes, Oggie, Thermo-Fisher |
| 10:30 a.m. |
Coffee Break |
| 11:00 a.m. |
Lab 4: Fluorescence Microscopy Labs: Protocols 14-17 |
| 12:45 p.m. |
Lunch (own your own)* |
| 1:30 p.m. |
Lecture 8: Introduction to Confocal/Multiphoton Microscopy - Basic Principles, Lemasters |
| 2:30 p.m. |
Group Photo (Anne Thompson) |
| 2:45 p.m. |
Coffee Break |
| 3:00 p.m. |
Lab 5: Confocal Labs: Protocols 18-20 |
| 6:00 p.m. |
Dinner (on your own) |
Wednesday, June 12
| Time |
Event |
| 8:30 a.m. |
Lecture 9: Optimizing Confocal Performance, Lemasters – VMB125 |
| 9:30 a.m. |
Coffee Break |
| 10:00 a.m. |
Lab 6: Confocal Lab: Protocols 21-22 |
| 12:00 p.m. |
Lunch* |
| 1:00 p.m. |
Keynote Lecture: Title TBA, Eeva-Liisa Eskelinen, Turku, Finland – BE112 |
| 2:00 p.m. to 5 p.m. |
Vendors Show DD 112 and Drug Discovery Lobby – Open to the Public |
| 2:30 p.m. |
Vendor presentations in VMB125 |
| 2:30 p.m. |
Deconvolution, Amy Jablonski, GE |
| 3:00 p.m. |
TBD, Oliver Tress, Zeiss |
| 6:00 p.m. |
Picnic Banquet in front of BE (Thermo Fisher) |
Thursday, June 13
| Time |
Event |
| 8:30 a.m. |
Lecture 10: FRET, FLIM and Advanced Fluorescence Techniques, Monika Gooz – VMB125 |
| 9:30 a.m. |
Coffee Break |
| 10:00 a.m. |
Lecture 11: Image analysis using FIJI/Image J, Thierry Pecot |
| 11:00 a.m. |
Lecture 12: Introducing Imaris image analysis software –James Shaw (Bitplane) |
| 12:00 p.m. |
Lunch* |
| 1:00 p.m. |
Lecture 13: Correlative Light-Electron Microscopy (CLEM), Eskelinen – VMB125 |
| 2:00 p.m. |
Coffee Break |
| 2:30 p.m. |
Lecture 14: Probing Living Cells and Tissues with Multiphoton Microscopy, Lemasters |
| 3:30 p.m. |
Lab 7: Multiphoton Microscopy: Protocols 23-26* |
| 6:00 p.m. |
Dinner (own your own) |
Friday, June 14
| Time |
Event |
| 8:30 a.m. |
Lecture 15: Super-resolution microscopy, Lemasters |
| 9:30 a.m. |
Coffee Break |
| 10:00 a.m. |
Lab 8 & 9: Super-resolution microscopy and intravital imaging demo (Zeiss Airyscan, Leica STED, Olympus 1200 MPE) |
| 12:00 p.m. |
Lunch-N-Learn seminar, Nancy Elliot, ibidi –VMB125 |
| 1:00 p.m. |
Lecture 16: Immunochemistry and Duolink, Gooz |
| 2:00 p.m. |
Lab 8 & 9: Super-resolution microscopy and intravital imaging demo (Zeiss Airyscan, Leica STED, Olympus 1200 MPE) |
| 3:00 p.m. |
Lecture 17: Scientific rigor and reproducibility – Eduardo Maldonado – VMB125 |
| 3:30 p.m. |
Certificates of Attendance and End of Course |
*Closest lunch options: MUSC main hospital cafeteria, ART cafeteria, Wickliffe house, HALO, and food trucks (in MUSC Horseshoe on Wednesday).
Five to ten min walk: Hominy Grill, Goulette Rotisserie, Fuel, R. Kitchen, Hardee’s, Subway
Vegan: Gnome Cafe
